Recombinant mouse monoclonal [r1032] antibody to MVP.
Spécificité
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Applications
WB, IHC-P
Contrôle positif
MCF-7 or HeLa cells. Human breast tumors.
Recommander des dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivité
Human
Immunogène
Proteins precipitated from human breast cancer MCF-7 cells.
Hôte
Mouse
Clonalité
Monoclonal
Clone
r1032
Isotype
IgG1
Chaînes légères
kappa
Conjuguer
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Masse moléculaire
104-110 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
LRP, Lung resistance related protein, Lung resistance-related protein, Major vault protein, Major vault protein, rat, homolog of, MVP_HUMAN, testicular secretory protein Li 30, VAULT 1, VAULT1