Munc 13-4 expression in Human T-lymphocyte lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Munc 13-4 Antibody (A84232) at 2µg/ml and detected by chemiluminescence.
Munc 13-4 expression in Human Thymus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Munc 13-4 Antibody (A84232) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
Munc 13-4 expression in Mouse Lung analyzed by immunohistochemistry. Tissue was paraffin-embedded. Staining was performed with Anti-Munc 13-4 Antibody (A84232) at 0.5µg/ml, wildtype (A) and knockout (B).
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