Recombinant Anti-Mucin 5AC Anticorps [MUC5AC/7066R] (A278619)

Anticorps monoclonal [MUC5AC/7066R] de lapin contre Mucin 5AC pour Flow Cytometry, IF, WB et IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Frais de livraison
Date de livraison
Livraison sous 4-6 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Anti-Mucin 5AC Antibody [MUC5AC/7066R] - BSA and Azide free
Description du produit
Recombinant rabbit monoclonal [MUC5AC/7066R] antibody to Mucin 5AC.
Spécificité
This antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb MUC5AC/7066R reacts with the protein backbone exclusively; it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then MUC5AC/7066R will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2.Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Applications
Flow Cytometry, IF, WB, IHC-P
Recommander des dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivité
Human
Réactivité croisée
This antibody is predicted to cross react with Monkey, Bovine, Feline, and Mouse.
Immunogène
Recombinant full-length human MUC5AC protein.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
MUC5AC/7066R
Isotype
IgG
Conjuguer

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Masse moléculaire
>1,000 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Notes générales
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Mucin 5AC Antibody [MUC5AC/7066R] (A278031).
Synonymes
Apomucin, Apomucin Major Airway Glycoprotein, Gastric mucin, LeB, Lewis B blood group antigen, Major Airway Glycoprotein, MUC 5, MUC5, MUC5AC, Mucin, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5 subtypes A and C tracheobronchial/gastric, Mucin 5, tracheobronchial/gastric, Mucin 5AC oligomeric mucus/gel forming, Mucin 5AC, oligomeric mucus/gel forming pseudogene, TBM, Tracheobronchial mucin, Tracheobronchial Mucin (TBM)
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Produits alternatifs à Anti-Mucin 5AC Anticorps [MUC5AC/7066R] (A278619)

Haut