MTOR (phospho Ser2448) Cell Based Kit Elisa (A103798)

Name: mTOR (phospho Ser2448) Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103798
Price: 645.0 USD
Availability: InStock

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$645

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Nom du produit

mTOR (phospho Ser2448) Cell Based ELISA Kit

Type de test

Cell-based (qualitative)

Principe du test

This mTOR (phospho Ser2448) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2448) and the effects that certain stimulation conditions have on mTOR (phospho Ser2448) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2448) concentration is achieved by an indirect ELISA format. In essence, mTOR (phospho Ser2448) is captured by Anti-mTOR (phospho Ser2448) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-mTOR Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Plate-forme

Microplate

Méthode de détection

Fluorometric

Gamme de detection

> 5000 Cells

Reactivité

Human, Mouse, Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at + 4°C. Stable for 6 months.

Synonymes

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, MTOR_HUMAN, OTTHUMP00000001983, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - mTOR (phospho Ser2448) Cell Based ELISA Kit (FLUO-CBP1620)

Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using Anti-mTOR (phospho Ser2448) Antibody.

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Figure 2: mTOR (phospho Ser2448) Cell Based ELISA Kit (FLUO-CBP1620)

A diagram showing the protocol for mTOR (phospho Ser2448) Cell Based ELISA Kit.

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Figure 3: mTOR (phospho Ser2448) Cell Based ELISA Kit (FLUO-CBP1620)

A summary of the protocol for mTOR (phospho Ser2448) Cell Based ELISA Kit.

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Figure 4: Western Blot - mTOR (phospho Ser2448) Cell Based ELISA Kit (FLUO-CBP1620)

The mouse monoclonal antibody to GAPDH is used as a positive control in mTOR (phospho Ser2448) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.