Anti-mTOR (phospho Ser2448) Anticorps (A10887)

Name: Anti-mTOR (phospho Ser2448) Antibody
Brand: Antibodies.com
SKU: A10887

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Ce produit est discontinué

Anti-mTOR (phospho Ser2448) Anticorps (A10887) a été abandonné et n'est plus disponible.

Voir toutes Anti-mTOR Anticorps.

Spécifications

Nom du produit

Anti-mTOR (phospho Ser2448) Antibody

Description du produit

Rabbit polyclonal antibody to mTOR (phospho Ser2448).

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S2448 of human mTOR (NP_004949.1).

Séquence

TDSYS

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

289 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, MTOR_HUMAN, OTTHUMP00000001983, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-mTOR (phospho Ser2448) Antibody (A10887)

Western blot analysis of extracts of 293T cells, using Anti-mTOR (phospho Ser2448) Antibody (A10887) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-mTOR (phospho Ser2448) Antibody (A10887)

Immunofluorescence analysis of HeLa cells using Anti-mTOR (phospho Ser2448) Antibody (A10887) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).