Anti-mTOR Antibody (A80535)

Name: Anti-mTOR Antibody
Brand: Antibodies.com
SKU: A80535
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to mTOR for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Ribosomal Marker

Specifications

Name

Anti-mTOR Antibody

Description

Rabbit polyclonal antibody to mTOR.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human mTOR (NP_004949.1).

Sequence

CHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

289 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-mTOR Antibody (A80535)

Western blot analysis of extracts of various cell lines, using Anti-mTOR Antibody (A80535) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunofluorescence - Anti-mTOR Antibody (A80535)

Immunofluorescence analysis of NIH-3T3 cells using Anti-mTOR Antibody (A80535) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-mTOR Antibody (A80535)

Immunofluorescence analysis of U-2 OS cells using Anti-mTOR Antibody (A80535) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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