Anti-mSin3A Antibody (A13518)

Name: Anti-mSin3A Antibody
Brand: Antibodies.com
SKU: A13518
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to mSin3A for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-mSin3A Antibody

Description

Rabbit polyclonal antibody to mSin3A.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:20-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Sin3A (NP_056292.1).

Sequence

MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Histone deacetylase complex subunit Sin3a, Paired amphipathic helix protein Sin3a, Transcriptional corepressor Sin3a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-mSin3A Antibody (A13518)

Western blot analysis of extracts of various cell lines, using Anti-mSin3A Antibody (A13518) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-mSin3A Antibody (A13518)

Immunofluorescence analysis of U2OS cells using Anti-mSin3A Antibody (A13518). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-mSin3A Antibody (A13518)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-mSin3A Antibody (A13518). This Western blot was performed on the immunoprecipitate using Anti-mSin3A Antibody (A13518) at a dilution of 1:1000.

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