Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.
Applications
WB, IHC-P
Contrôle positif
A549 or HepG2 cells. Human colon, thyroid, testis or lymph node.
Recommander des dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivité
Human
Immunogène
Recombinant fragment (around Amino Acids 300-500) of human MSH2 protein. The exact sequence is proprietary.
Hôte
Mouse
Clonalité
Monoclonal
Clone
MSH2/3165
Isotype
IgG2
Chaînes légères
kappa
Conjuguer
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Masse moléculaire
100 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC, HNPCC 1, HNPCC1, LCFS2, MSH 2, MSH2_HUMAN, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2