Souris Leptin Kit Elisa (A643)

$635
Frais de livraison
Date de livraison
Livraison sous 7-10 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Mouse Leptin ELISA Kit
Type de test
Sandwich (quantitative)
Principe du test
This Leptin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Leptin Antibody. Standards or samples are then added to the microtire plate wells and Leptin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Leptin present in the sample, Anti-Leptin Antibody (Biotin) is added to each well to "sandwich" the Leptin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Leptin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensibilité
< 10 pg/ml
Gamme de detection
62.5-4000 pg/ml
Reactivité
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonymes
FLJ94114, LEP, LEPD, Leptin (murine obesity homolog), Leptin (obesity homolog, mouse), Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, LEP_HUMAN, OB, Obese protein, Obese, mouse, homolog of, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS, OTTHUMP00000212285
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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