Mouse IL-33 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-33 in the samples. A monoclonal antibody specific for mouse IL-33 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IL-33, and IL-33 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-33 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

8.74 pg/ml

Gamme de detection

46.88 - 3000 pg/ml

Récupération

98% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant mouse IL-33.

Reactivité

Mouse

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

C9orf26, CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27, DVS27 related protein, IL 1F11, IL 33, IL-1F11, IL1F11, IL33, IL33_HUMAN, Interleukin 1 family member 11, Interleukin 33, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin-1 family member 11, Interleukin-33, Interleukin-33 (109-270), Interleukin33, NF HEV, NF-HEV, NFEHEV, NFHEV, Nuclear factor for high endothelial venules, Nuclear factor from high endothelial venules, OTTHUMP00000021041, RP11 575C20.2, RP11-575C20.2

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.