Mouse IL-27 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-27 in the samples. A monoclonal antibody specific for mouse IL-27 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and IL-27 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for IL-27 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-27 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
1.48 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Récupération
91% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant mouse IL-27.
Reactivité
Mouse
Réactivité croisée
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2 - 8°C.
Synonymes
IL 17D, IL 22, IL 27, IL-17D, IL17D, IL17D_HUMAN, IL27, Interleukin 17D, Interleukin 27, Interleukin-17D, Interleukin-27
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
