Mouse IL-23 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-23 in the samples. A monoclonal antibody specific for mouse IL-23 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and IL-23 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for IL-23 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-23 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

3.10 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Récupération

91% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant mouse IL-23.

Reactivité

Mouse

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23 subunit alpha, IL-23-A, IL-23p19, IL12B, IL23, Il23a, IL23A_HUMAN, IL23P19, interleukin 12B, Interleukin 23 alpha subunit p19, Interleukin 23 p19 subunit, interleukin 23 subunit alpha, interleukin 23 subunit p19, interleukin six, G CSF related factor, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T cell activation, MGC79388, P19, SGRF

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.