Mouse IL-17F ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-17F in the samples. A monoclonal antibody specific for mouse IL-17F has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-17F, and IL-17F present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17F bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
0.59 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Récupération
106% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant mouse IL-17F.
Reactivité
Mouse
Réactivité croisée
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2 - 8°C.
Synonymes
CANDF6, Cytokine ML 1, Cytokine ML-1, IL 17F, IL 24, IL-24, IL17F, IL17F_HUMAN, Interleukin 17F, Interleukin 24, Interleukin-17F, Interleukin-24, ML 1, ML1, Mutant IL 17F, OTTHUMP00000016602
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
