Figure 1: Anti-Mouse IgA Antibody [RM220] (Biotin) (A121268)
ELISA of mouse immunoglobulins shows Anti-Mouse IgA Antibody [RM220] reacts to mouse IgA. There is no cross reactivity with mouse IgG1, IgG2a,IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgA Antibody [RM220] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Figure 2: Anti-Mouse IgA Antibody [RM220] (Biotin) (A121268)
A titer ELISA of mouse IgA. The plate was coated with different amounts of mouse IgA. A serial dilution of Anti-Mouse IgA Antibody [RM220] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Figure 3: Western Blot - Anti-Mouse IgA Antibody [RM220] (Biotin) (A121268)
Western blot of non-reduced (-) and reduced (+) mouse IgA, using 0.5µg/ml of Anti-Mouse IgA Antibody [RM220] (Biotin). This antibody reacts to non-reduced IgA.