Mouse Granzyme B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Granzyme B in the samples. A monoclonal antibody specific for mouse Granzyme B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Granzyme B, and Granzyme B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Granzyme B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

9.91 pg/ml

Gamme de detection

312.50 - 20000 pg/ml

Récupération

99% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant mouse Granzyme B.

Reactivité

Mouse

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

C11, Cathepsin G like 1, Cathepsin G-like 1, CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA-1, CTLA1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T lymphocyte proteinase 2, Cytotoxic T-lymphocyte proteinase 2, Fragmentin 2, Fragmentin-2, GRAB_HUMAN, Granzyme 2, Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1), Granzyme-2, GranzymeB, GRB, Gzmb, Hlp, Human lymphocyte protein, Lymphocyte protease, Protease, serine, B, SECT, T cell serine protease 1-3E, T-cell serine protease 1-3E

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.