Principe du test
Mouse GALC ELISA Kit (A311003) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse GALC in serum, plasma or other biological fluids. An antibody specific for GALC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GALC present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-GALC Antibody, which also binds the GALC present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-GALC Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-GALC Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of GALC captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of GALC in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.