Anti-MMP1 Anticorps [F09-3C7] (Biotin) (A280391)

Anti-MMP1 Antibody [F09-3C7] (Biotin)

Mouse monoclonal [F09-3C7] antibody to MMP1 (Biotin).

This antibody recognises matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase. MMP-1 is a 45 kDa protein in its active form and is a member of the metzincins superfamily of proteases. It is produced by various cells and tissues including the endothelium, fibroblasts, smooth muscle cells, vascular adventitia and platelets. MMP-1 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the pro-MMP-1. This is then further cleaved by proteolytic enzymes to create the active form. Like other MMP’s, MMP-1 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-1 degrades the collagen proteins type I, II, III, VII, VIII, X and gelatin. It is also able to target other ECM substrates including aggrecan, nidogen and perlecan. MMP1 expression levels are low in most cells under physiological conditions, but are upregulated during inflammatory conditions and autoimmune diseases. For example a single nucleotide polymorphism of MMP-1 at the promoter region has been associated with an increased risk of sudden deafness linked to circulatory disturbance and inflammation. The biotinylated Mouse anti Human MMP-1 antibody, clone F09-3C7 (MCA6255B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-1 antibody, clone F08-2F1 (MCA6254GA) as the capture antibody.

ELISA

ELISA: 1 µg/ml

Human

E. coli derived recombinant human partial length of MMP-1 (Phe100-Thr274).

Mouse

Monoclonal

F09-3C7

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human MMP-1 antibody, clone F09-3C7, recognizes matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase. MMP-1 is a 45 kDa protein in its active form and is a member of the metzincins superfamily of proteases. It is produced by various cells and tissues including the endothelium, fibroblasts, smooth muscle cells, vascular adventitia and platelets (Cui et al. 2017). MMP-1 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the pro-MMP-1. This is then further cleaved by proteolytic enzymes to create the active form. Like other MMP’s, MMP-1 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-1 degrades the collagen proteins type I, II, III, VII, VIII, X and gelatin. It is also able to target other ECM substrates including aggrecan, nidogen and perlecan (Cui et al. 2017). MMP1 expression levels are low in most cells under physiological conditions, but are upregulated during inflammatory conditions and autoimmune diseases (Mittal et al. 2016). For example a single nucleotide polymorphism of MMP-1 at the promoter region has been associated with an increased risk of sudden deafness linked to circulatory disturbance and inflammation (Nam et al. 2011). The biotinylated Mouse anti Human MMP-1 antibody, clone F09-3C7 (MCA6255B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-1 antibody, clone F08-2F1 (MCA6254GA) as the capture antibody.

27 kDa interstitial collagenase, CLG, CLGN , collagenase, fibroblast, collagenase, interstitial, Fibroblast collagenase, Interstitial collagenase , Matrix metallopeptidase 1, Matrix metallopeptidase 1 (interstitial collagenase), Matrix metalloprotease 1, Matrix Metalloproteinase 1 , Matrix metalloproteinase-1, MMP 1 , MMP-1, MMP1_HUMAN, OTTHUMP00000045866

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.