Anti-MLH1 Anticorps [ARC53543] (A308155)

Name: Anti-MLH1 Antibody [ARC53543]
Brand: Antibodies.com
SKU: A308155
Price: 470.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$470

Anticorps monoclonal [ARC53543] de lapin contre MLH1 pour WB et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-MLH1 Antibody [ARC53543]

Description du produit

Rabbit monoclonal [ARC53543] antibody to MLH1.

Applications

WB, IHC

Recommander des dilutions

WB: 1:10,000-1:70,000, IHC: 1:50-1:200

Reactivité

Human, Mouse

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1 (NP_000240.1).

Séquence

PGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGD

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC53543

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

85 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of various cell lines, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of mouse brain, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts from normal (control) and MLH1 knockout (KO) HeLa cells, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [ARC53543] (A308155)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control sample) using Anti-MLH1 Antibody [ARC53543] (A308155) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.