Anti-MEK1 (phospho Ser298) Anticorps (A16425)

Name: Anti-MEK1 (phospho Ser298) Antibody
Brand: Antibodies.com
SKU: A16425
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre MEK1 (phospho Ser298) pour WB et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-MEK1 (phospho Ser298) Antibody

Description du produit

Rabbit polyclonal antibody to MEK1 (phospho Ser298).

Applications

WB, IP

Recommander des dilutions

WB: 1:500-1:2,000, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S298 of human MEK1 (NP_002746.1).

Séquence

PLSSY

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

43 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Western blot analysis of extracts of various cell lines, using Anti-MEK1 (phospho Ser298) Antibody (A16425) at 1:1,000 dilution or MEK1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Western blot analysis of extracts of various cell lines, using Anti-MEK1 (phospho Ser298) Antibody (A16425) at 1:1,000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-MEK1 (phospho Ser298) Antibody (A16425). This Western blot was performed on the immunoprecipitate using Anti-MEK1 (phospho Ser298) Antibody (A16425) at a dilution of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.