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MED26 Cell Based Kit Elisa (A103467)

Nom du produit
MED26 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This MED26 Cell Based ELISA Kit allows for the detection of MED26 and the effects that certain stimulation conditions have on MED26 expression in different cell lines. Qualitative determination of MED26 concentration is achieved by an indirect ELISA format. In essence, the MED26 is captured by Anti-MED26 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MED26 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Activator recruited cofactor 70 kDa component, Activator-recruited cofactor 70 kDa component, ARC 70, ARC70, Cofactor required for Sp1 transcriptional activation subunit 7, Cofactor required for Sp1 transcriptional activation, subunit 7 (70kD), Cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa, CRSP 7, CRSP complex subunit 7, CRSP7, CRSP70, MED 26, MED26_HUMAN, Mediator complex subunit 26, Mediator of RNA polymerase II transcription subunit 26, Transcriptional coactivator CRSP70
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - MED26 Cell Based ELISA Kit (CB5820)
Western blot analysis of extracts from LOVO cells, using Anti-MED26 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: MED26 Cell Based ELISA Kit (CB5820)
A diagram showing the protocol for MED26 Cell Based ELISA Kit.
Protocol Summary - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: MED26 Cell Based ELISA Kit (CB5820)
A summary of the protocol for MED26 Cell Based ELISA Kit.
Western Blot - Control Antibody - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - MED26 Cell Based ELISA Kit (CB5820)
The mouse monoclonal antibody to GAPDH is used as a positive control inMED26 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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