Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP: IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

Western blot analysis of mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: mouse Cortex. Lane 3: mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MDC1 Antibody [P2B11] (A304945), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.