Synthetic peptide within amino acids 1375-1425 of human MDC1 (NP_055456. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
KIAA0170, Mediator of DNA damage checkpoint protein 1, NFBD1, Nuclear factor with BRCT domains 1
Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Anti-MDC1 Antibody (A295223) was used at 1 µg/ml for WB and used for IP at 3 µg/mg lysate. MDC1 was also immunoprecipitated by another lot of Anti-MDC1 Antibody (A295223). Detection: Chemiluminescence with exposure time of 3 seconds.
Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Anti-MDC1 Antibody (A295223) was used at 0.1 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.
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