Anti-MDA5 Antibody (A88241)

Name: Anti-MDA5 Antibody
Brand: Antibodies.com
SKU: A88241
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to MDA5 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-MDA5 Antibody

Description

Rabbit polyclonal antibody to MDA5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 695-865 of human IFIH1 (NP_071451.2).

Sequence

EYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CADM-140 autoantigen, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicard, Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, Interferon-induced with helicase C domain protein 1, MDA-5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-MDA5 Antibody (A88241)

Western blot analysis of extracts of BxPC-3 cells, using Anti-MDA5 Antibody (A88241) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-MDA5 Antibody (A88241)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-MDA5 Antibody (A88241) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MDA5 Antibody (A88241)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-MDA5 Antibody (A88241) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MDA5 Antibody (A88241)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-MDA5 Antibody (A88241) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.