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MASTL Cell Based Kit Elisa (A103490)

Nom du produit
MASTL Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This MASTL Cell Based ELISA Kit allows for the detection of MASTL and the effects that certain stimulation conditions have on MASTL expression in different cell lines. Qualitative determination of MASTL concentration is achieved by an indirect ELISA format. In essence, the MASTL is captured by Anti-MASTL Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MASTL Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
2700091H24Rik, C88295, FLJ14813, GREATWALL, Greatwall protein kinase, GW, GWL, GWL_HUMAN, hGWL, MAST-L, MGC117975, Microtubule associated serine/threonine kinase like, Microtubule-associated serine/threonine-protein kinase-like, RP11 85G18.2, Serine/threonine-protein kinase greatwall, THC2
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - MASTL Cell Based ELISA Kit (CB6111) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - MASTL Cell Based ELISA Kit (CB6111)
Western blot analysis of extracts from HepG2 cells, using Anti-MASTL Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - MASTL Cell Based ELISA Kit (CB6111) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: MASTL Cell Based ELISA Kit (CB6111)
A diagram showing the protocol for MASTL Cell Based ELISA Kit.
Protocol Summary - MASTL Cell Based ELISA Kit (CB6111) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: MASTL Cell Based ELISA Kit (CB6111)
A summary of the protocol for MASTL Cell Based ELISA Kit.
Western Blot - Control Antibody - MASTL Cell Based ELISA Kit (CB6111) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - MASTL Cell Based ELISA Kit (CB6111)
The mouse monoclonal antibody to GAPDH is used as a positive control inMASTL Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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