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MARK3 Cell Based Kit Elisa (A103142)

Nom du produit
MARK3 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This MARK3 Cell Based ELISA Kit allows for the detection of MARK3 and the effects that certain stimulation conditions have on MARK3 expression in different cell lines. Qualitative determination of MARK3 concentration is achieved by an indirect ELISA format. In essence, the MARK3 is captured by Anti-MARK3 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MARK3 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
C-TAK1, Cdc25C associated protein kinase 1, Cdc25C-associated protein kinase 1, cTAK1, ELKL motif kinase 2, EMK-2, Emk2, ETK 1, KIAA4230, KP78, MAP microtubule affinity regulating kinase 3, MAP/microtubule affinity-regulating kinase 3, MARK3_HUMAN, Par 1a, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine threonine protein kinase p78, Serine/threonine-protein kinase p78, SerThr protein kinase PAR 1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - MARK3 Cell Based ELISA Kit (CB5865) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - MARK3 Cell Based ELISA Kit (CB5865)
Western blot analysis of extracts from HeLa/293 cells, using Anti-MARK3 Antibody.
Protocol Diagram - MARK3 Cell Based ELISA Kit (CB5865) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: MARK3 Cell Based ELISA Kit (CB5865)
A diagram showing the protocol for MARK3 Cell Based ELISA Kit.
Protocol Summary - MARK3 Cell Based ELISA Kit (CB5865) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: MARK3 Cell Based ELISA Kit (CB5865)
A summary of the protocol for MARK3 Cell Based ELISA Kit.
Western Blot - Control Antibody - MARK3 Cell Based ELISA Kit (CB5865) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - MARK3 Cell Based ELISA Kit (CB5865)
The mouse monoclonal antibody to GAPDH is used as a positive control inMARK3 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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