Anti-Mannose Receptor Anticorps [MR5D3] (PE) (A281280)

Anti-Mannose Receptor Antibody [MR5D3] (PE)

Rat monoclonal [MR5D3] antibody to Mannose Receptor (PE).

This antibody recognises the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells. The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognises carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins. Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in western blotting (Martinez-Pomares et al. 2003 and Su et al. 2005).

Flow Cytometry

FC 1: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

Chimaeric CRD4-7-Fc protein.

Rat

Monoclonal

MR5D3

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002).The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005).Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in western blotting (Martinez-Pomares et al. 2003 and Su et al. 2005).

bA541I19.1, C type lectin domain family 13 member D, C-type lectin domain family 13 member D, CD 206, CD206, CD206 antigen, CLEC13D, CLEC13DL, Macrophage mannose receptor, Macrophage mannose receptor 1, Macrophage mannose receptor 1 like protein 1, Mannose receptor C type 1, Mannose receptor C type 1 like 1, MMR, MRC 1, MRC1, MRC1L1, MRC1_HUMAN, OTTHUMP00000045206

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.