Anti-LRP6 (phospho Ser1490) Antibody (A308216)

Western blot analysis of extracts of HeLa cells, using Anti-LRP6 (phospho Ser1490) Antibody (A308216) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.