Anti-Lipoamide Dehydrogenase Antibody (A90439)

Name: Anti-Lipoamide Dehydrogenase Antibody
Brand: Antibodies.com
SKU: A90439
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Lipoamide Dehydrogenase for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Lipoamide Dehydrogenase Antibody

Description

Rabbit polyclonal antibody to Lipoamide Dehydrogenase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2).

Sequence

ADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, mitochondrial, GCSL, Glycine cleavage system L protein, LAD, PHE3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Lipoamide Dehydrogenase Antibody (A90439)

Western blot analysis of extracts of various cell lines, using Anti-Lipoamide Dehydrogenase Antibody (A90439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody (A90439)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Lipoamide Dehydrogenase Antibody (A90439) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody (A90439)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Lipoamide Dehydrogenase Antibody (A90439) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.