Anti-Leptin Receptor Anticorps (A83422)

Name: Anti-Leptin Receptor Antibody
Brand: Antibodies.com
SKU: A83422
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab50424). Découvrez plus pour moins cher.

Cliquez pour ouvrir le point de vue élargi

$480

Anticorps polyclonal de chèvre contre Leptin Receptor pour ELISA, WB, IHC et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Leptin Receptor Antibody

Description du produit

Goat polyclonal antibody to Leptin Receptor.

Applications

ELISA, WB, IHC, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 1-2µg/ml, IHC: 4-6µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human, Mouse, Rat

Immunogène

Synthetic peptide corresponding to the internal region of human Leptin Receptor.

Séquence

C-TQDDIEKHQSDAG

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

140-150 kDa

MW prédit

132kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

CD 295, CD295, CD295 antigen, Db, Fa, HuB219, LEP R, LEP-R, LEPR, LEPRD, LEPR_HUMAN, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein, OB receptor, OB-R, OB-RGRP, obl, Obr

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Cerebellum (A), Mouse Foetal Brain (B), and Rat Brain (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Leptin Receptor Antibody (A83422) at 1.75µg/ml (A) or 1.5µg/ml (B-C) and detected by chemiluminescence.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 6µg/ml and revealed with horseradish peroxidase (HRP).

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Negative control for Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Flow Cytometry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.