Anti-Laminin beta 1 Antibody (A305674)

Name: Anti-Laminin beta 1 Antibody
Brand: Antibodies.com
SKU: A305674

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This product is discontinued

Anti-Laminin beta 1 Antibody (A305674) has been discontinued and is no longer available.

View all Laminin beta 1 Antibodies.

Specifications

Name

Anti-Laminin beta 1 Antibody

Description

Rabbit polyclonal antibody to Laminin beta 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).

Sequence

EAKLRADEAKQSAEDILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAEDVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVYSTCL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

LAMB1, Laminin B1 chain, Laminin subunit beta-1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Laminin beta 1 Antibody (A305674)

Western blot analysis of extracts of various cell lines, using Anti-Laminin beta 1 Antibody (A305674) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-Laminin beta 1 Antibody (A305674)

Immunofluorescence analysis of L929 cells using Anti-Laminin beta 1 Antibody (A305674) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).