Figure 1: Western Blot - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Western blot analysis of extracts from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using Anti-Lamin A Antibody [ARC5001-08] (A308800) at 1:50,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Western blot analysis of extracts of various cell lines, using Anti-Lamin A Antibody [ARC5001-08] (A308800) at 1:500000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Figure 3: Immunohistochemistry - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 4: Immunohistochemistry - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:400 - 1:3200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 5: Immunofluorescence - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunofluorescence analysis of H9C2 cells using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
Figure 6: Immunofluorescence - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunofluorescence analysis of L929 cells using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
Figure 7: Immunofluorescence - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Confocal immunofluorescence analysis of HeLa cells using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Figure 8: Immunofluorescence - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunofluorescence analysis of U2OS cells using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of 1:100 - 1:1600. DAPI was used to stain the cell nuclei (blue).
Figure 9: Western Blot - Anti-Lamin A Antibody [ARC5001-08] (A308800)
Immunoprecipitation analysis of 300µg extracts of PC-12 cells using 3µg of Anti-Lamin A Antibody [ARC5001-08] (A308800). This Western blot was performed on the immunoprecipitate using Anti-Lamin A Antibody [ARC5001-08] (A308800) at a dilution of1:100000.