Anti-Lamin A Antibody (A8408)

Name: Anti-Lamin A Antibody
Brand: Antibodies.com
SKU: A8408
Price: 345.0 USD
Availability: InStock

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This product is discontinued

Anti-Lamin A Antibody (A8408) has been discontinued and is no longer available.

View all Lamin A Antibodies.

Specifications

Name

Anti-Lamin A Antibody

Description

Rabbit polyclonal antibody to Lamin A.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_005563.1).

Sequence

SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHVSGSRR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa / 72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glyercol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

LMN1, LMNA, Prelamin-A/C

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-Lamin A Antibody (A8408)

Western blot analysis of various lysates, using Anti-Lamin A Antibody (A8408) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Lamin A Antibody (A8408)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Lamin A Antibody (A8408) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Lamin A Antibody (A8408)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Lamin A Antibody (A8408) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Lamin A Antibody (A8408)

Immunofluorescence analysis of A-549 cells using Anti-Lamin A Antibody (A8408) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).