Figure 1: Western Blot - Anti-Lamin A Antibody (A12573)
Western blot analysis of various lysates, using Anti-Lamin A Antibody (A12573) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Immunohistochemistry - Anti-Lamin A Antibody (A12573)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Lamin A Antibody (A12573) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 3: Immunohistochemistry - Anti-Lamin A Antibody (A12573)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Lamin A Antibody (A12573) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 4: Immunohistochemistry - Anti-Lamin A Antibody (A12573)
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Lamin A Antibody (A12573) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 5: Immunofluorescence - Anti-Lamin A Antibody (A12573)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Lamin A Antibody (A12573) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).