This antibody recognises mouse lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognises an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II.
Applications
Flow Cytometry, Functional Assays
Recommander des dilutions
Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivité
Mouse
Immunogène
Murine CD223 Ig fusion protein.
Hôte
Rat
Clonalité
Monoclonal
Clone
C9B7W
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Stockage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Notes générales
Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).
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