Anti-Ku80 Anticorps [ARC0706] (A307476)

Name: Anti-Ku80 Antibody [ARC0706]
Brand: Antibodies.com
SKU: A307476
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0706] de lapin contre Ku80 pour WB, IHC, ICC/IF et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Ku80 Antibody [ARC0706]

Description du produit

Rabbit monoclonal [ARC0706] antibody to Ku80.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010).

Séquence

MKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0706

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

86 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II, ATP dependent DNA helicase II 80 kDa subunit, ATP dependent DNA helicase II 86 Kd subunit, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box binding factor 85 kDa, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP 1, KARP1, Ku 80, Ku autoantigen 80kDa, Ku86, Ku86 autoantigen related protein 1, KUB 2, KUB2, Lupus Ku autoantigen protein p86, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining), X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5, Xray repair complementing defective repair in Chinese hamster cells 5, XRCC 5, XRCC5, XRCC5_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-Ku80 Antibody [ARC0706] (A307476)

Western blot analysis of extracts of various cell lines, using Anti-Ku80 Antibody [ARC0706] (A307476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Ku80 Antibody [ARC0706] (A307476)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Ku80 Antibody [ARC0706] (A307476) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Ku80 Antibody [ARC0706] (A307476)

Confocal imaging of HeLa cells using Anti-Ku80 Antibody [ARC0706] (A307476), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 4: Western Blot - Anti-Ku80 Antibody [ARC0706] (A307476)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Ku80 Antibody [ARC0706] (A307476). This Western blot was performed on the immunoprecipitate using Anti-Ku80 Antibody [ARC0706] (A307476) at a dilution of 1:1000.