Figure 1: Western Blot - Anti-KIF4A/KIF4 Antibody (A83849)
KIF4A/KIF4 expression in NIH3T3 nuclear (A) and negative control Human Ovary (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-KIF4A/KIF4 Antibody (A83849) at 2µg/ml and detected by chemiluminescence.
KIF4A/KIF4 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-KIF4A/KIF4 Antibody (A83849) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
KIF4A/KIF4 expression in HEK293 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-KIF4A/KIF4 Antibody (A83849) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
