Rabbit polyclonal antibody to Keratin 18 (phospho Ser52).
Spécificité
This antibody detects endogenous levels of Keratin 18 only when phosphorylated at Ser52.
Applications
WB, IF, ELISA
Recommander des dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivité
Human
Immunogène
Synthetic peptide derived from human Keratin 18 around the phosphorylation site of Ser52 (amino acids 21-70).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Masse moléculaire
48kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CK18, CK19, CK8, K18, K19, K8, keratin 18, keratin 19, keratin 8, Keratin type I 40kDa, Keratin type I cytoskeletal 18, Keratin type I cytoskeletal 19, Keratin type II cytoskeletal 8, KRT18, KRT19, KRT8
Figure 1: Western Blot - Anti-Keratin 18 (phospho Ser52) Antibody (A94696)
Western blot analysis of lysates from HepG2 cells using Anti-Keratin 18 (phospho Ser52) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Keratin 18 (phospho Ser52) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human brain using Anti-Keratin 18 (phospho Ser52) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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