Anti-KAP1 Anticorps (A295397)

Name: Anti-KAP1 Antibody
Brand: Antibodies.com
SKU: A295397
Price: 525.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab10484). Découvrez plus pour moins cher.

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$525

Anticorps polyclonal de lapin contre KAP1 pour WB, IP et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-KAP1 Antibody

Description du produit

Rabbit polyclonal antibody to KAP1.

Applications

WB, IP, IHC

Recommander des dilutions

IHC: 1:1,000-1:5,000, IP: 2-10 µg/1 mg lysate, WB: 1:2,000-1:10,000

Reactivité

Human, Mouse

Immunogène

Synthetic peptide within amino acids 650-700 of human KAP1 (NP_005753. 1).

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Notes générales

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonymes

E3 SUMO protein ligase TRIM28, E3 SUMO-protein ligase TRIM28, FLJ29029, KAP 1, KAP-1, KRAB associated protein 1, KRAB interacting protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP 1, KRIP-1, KRIP1, Nuclear corepressor KAP 1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1-beta, TIF1B, TIF1B_HUMAN, Transcription intermediary factor 1 beta, Transcription intermediary factor 1-beta, Trim28, Tripartite motif containing 28, tripartite motif containing protein 28, Tripartite motif-containing protein 28

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunohistochemistry - Anti-KAP1 Antibody (A295397)

Sample: FFPE section of mouse plasmacytoma. Antibody: Anti-KAP1 Antibody (A295397) was used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

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Figure 2: Immunohistochemistry - Anti-KAP1 Antibody (A295397)

Sample: FFPE section of human ovarian carcinoma. Antibody: Anti-KAP1 Antibody (A295397) was used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

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Figure 3: Immunoprecipitation - Anti-KAP1 Antibody (A295397)

Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-KAP1 Antibody (A295397) was used for IP at 6 µg per reaction. KAP1 was also immunoprecipitated by a previous lot of this antibody and another rabbit anti-KAP1 antibody. For blotting immunoprecipitated KAP1, Anti-KAP1 Antibody (A295397) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Figure 4: Western Blot - Anti-KAP1 Antibody (A295397)

Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-KAP1 Antibody (A295397) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.