Mouse monoclonal [PCRP-JAZF1-1C2] antibody to JAZF1.
Spécificité
JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.
JAZF zinc finger 1, JAZF1_HUMAN, Juxtaposed with another zinc finger protein 1, TAK1 interacting protein 27, TAK1-interacting protein 27, TIP27, Zinc finger protein 802, ZNF802
SDS-PAGE analysis of Anti-JAZF1 Antibody [PCRP-JAZF1-1C2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-JAZF1 Antibody [PCRP-JAZF1-1C2] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).
Flow cytometric analysis of PFA-fixed HeLa cells using Anti-JAZF1 Antibody [PCRP-JAZF1-1C2] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (Red).
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-JAZF1 Antibody [PCRP-JAZF1-1C2]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.