Figure 1: Western Blot - Anti-ITK/EMT Antibody (A83776)
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with Anti-ITK Antibody (A83776) (1µg/ml) in Lane A and probed with Anti-DYKDDDDK Tag Antibody (1/10000) in Lane C. Mock-transfected HEK293 probed with Anti-ASNSD1 Antibody (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.