Anti-IRS1 Anticorps [IRS1/7571] (A316580)

$765

Anticorps monoclonal [IRS1/7571] de souris contre IRS1 pour IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Frais de livraison
Date de livraison
Livraison sous 4-6 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Anti-IRS1 Antibody [IRS1/7571] - BSA and Azide free
Description du produit
Mouse monoclonal [IRS1/7571] antibody to IRS1.
Spécificité
The Insulin receptor substrate-1 (IRS-1), a protein major substrate of the Insulin receptor, is phosphorylated in response to stimulation of cells by Insulin, Insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An Insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the Insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase IIcatalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase IIcatalyzed phosphorylation of IRS-1 may be a component of the intracellular Insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the Insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of Insulin sensitivity by interrupting the association between the Insulin receptor and IRS-1.
Applications
IHC-P
Contrôle positif
Human breast carcinoma, adrenal gland or lung.
Recommander des dilutions
IHC: 1-2 µg/ml
Reactivité
Human
Immunogène
Recombinant fragment (around Amino Acids 1-200) of human IRS1 protein. The exact sequence is proprietary.
Hôte
Mouse
Clonalité
Monoclonal
Clone
IRS1/7571
Isotype
IgG2
Chaînes légères
kappa
Conjuguer

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Masse moléculaire
170-185 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1_HUMAN, OTTHUMP00000164234
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Produits alternatifs à Anti-IRS1 Anticorps [IRS1/7571] (A316580)

Haut