Anti-IRGM Anticorps (A304839)

Name: Anti-IRGM Antibody
Brand: Antibodies.com
SKU: A304839
Price: 500.0 USD
Availability: InStock

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$500

Anticorps polyclonal de lapin contre IRGM pour WB, ICC/IF et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-IRGM Antibody

Description du produit

Rabbit polyclonal antibody to IRGM.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivité

Human

Immunogène

Synthetic peptide from the N-terminal of Human IRGM.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Masse moléculaire

~20 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

IFI1, Iigp3, Iipg3, Immunity related GTPase family M protein 1, Immunity related GTPase family, M, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1, Interferon inducible protein 1, Interferon-inducible protein 1, IRGM1, IRGM_HUMAN, LPS-stimulated RAW 264.7 macrophage protein 47 homolog, LRG 47, LRG-47, LRG-47-like protein, LRG47, MGC149263, MGC149264

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunohistochemistry - Anti-IRGM Antibody (A304839)

Immunohistochemistry analysis of human colon cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-IRGM Antibody (A304839) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

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Figure 2: Immunohistochemistry - Anti-IRGM Antibody (A304839)

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Figure 3: Western Blot - Anti-IRGM Antibody (A304839)

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Anti-IRGM Antibody (A304839) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~20.1 kDa.

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Figure 4: Immunofluorescence - Anti-IRGM Antibody (A304839)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-IRGM Antibody (A304839), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.