Anti-IRAK4 Anticorps (A84059)

Name: Anti-IRAK4 Antibody
Brand: Antibodies.com
SKU: A84059
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab113525). Découvrez plus pour moins cher.

Cliquez pour ouvrir le point de vue élargi

$480

Anticorps polyclonal de chèvre contre IRAK4 pour ELISA, WB, IHC, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-IRAK4 Antibody

Description du produit

Goat polyclonal antibody to IRAK4.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:32,000, WB: 0.5-2µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the N terminal region of human IRAK4.

Séquence

NKPITPSTYVRC

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

50 kDa

MW prédit

51.5kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

IL-1 receptor-associated kinase 4, Interleukin 1 receptor associated kinase 4 mutant form 1, Interleukin-1 receptor-associated kinase 4, Interleukin1 receptor associated kinase 4, IPD1, IRAK 4, IRAK-4, IRAK4 mutated form 1, IRAK4_HUMAN, LOC 51135, NY REN 64, NY REN 64 antigen, NY-REN-64, REN64, Renal carcinoma antigen NY-REN-64

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa (A) and Jurkat (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-IRAK4 Antibody (A84059) at 1µg/ml and detected by chemiluminescence.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunofluorescence - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Flow Cytometry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IRAK4 Antibody (A84059) at 3.75µg/ml and revealed with alkaline phosphatase (AP).