Rabbit polyclonal antibody to Insulin degrading enzyme / IDE.
Applications
WB, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, ICC/IF: 1:10-1:100
Reactivité
Human, Mouse
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) (NP_004960.2).
Figure 1: Western Blot - Anti-Insulin degrading enzyme / IDE Antibody (A13561)
Western blot analysis of extracts of various cell lines, using Anti-Insulin degrading enzyme / IDE Antibody (A13561) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.
Figure 2: Western Blot - Anti-Insulin degrading enzyme / IDE Antibody (A13561)
Western blot analysis of extracts from normal (control) and Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) knockout (KO) HeLa cells, using Anti-Insulin degrading enzyme / IDE Antibody (A13561) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
![Western Blot - Anti-Insulin degrading enzyme / IDE Antibody [ARC0537] (A307705) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307705_1.jpg?profile=product_alternative)
