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Anti-Virus de la Grippe B Hemagglutinin Anticorps [1H12] (A337)

Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region.. Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.
$535

Anticorps monoclonal [1H12] de souris contre Influenza B Virus Hemagglutinin pour WB, IP, ICC/IF et ELISA.

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Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

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+1 (314) 370-6046
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Nom du produit
Anti-Influenza B Virus Hemagglutinin Antibody [1H12]
Description du produit
Mouse monoclonal (1H12) antibody to Influenza B Virus Hemagglutinin.
Applications
WB, IP, ICC/IF, ELISA
Recommander des dilutions
WB: 1:500-1:1,000, ICC/IF: 1:100-1:200, IP: 1:200
Réactivité croisée
This antibody does not react with Influenza A viruses.
Immunogène
Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.
Hôte
Mouse
Clonalité
Monoclonal
Clone
1H12
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (65-160)
Anti-Influenza B Virus Hemagglutinin Antibody
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.Cliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (65-160)
Anti-Influenza B Virus Hemagglutinin Antibody

Produits alternatifs à Anti-Virus de la Grippe B Hemagglutinin Anticorps [1H12] (A337)

Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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