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Anti-Virus de la Grippe A Nucleoprotein Anticorps [C43] (A335)

Ce produit est identique à Abcam (ab128193). Découvrez plus pour moins cher.

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HA Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses. Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.
$535

Anticorps monoclonal [C43] de souris contre Influenza A Virus Nucleoprotein pour WB, IP, IF, IHC et ELISA.

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Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

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Nom du produit
Anti-Influenza A Virus Nucleoprotein Antibody [C43]
Description du produit
Mouse monoclonal (C43) antibody to Influenza A Virus Nucleoprotein.
Spécificité
This antibody reacts with NP of all Influenza A viruses tested so far, including seasonal H2N2, H3N2, and avian H5N1, H5N2, and H1N1 (seasonal, pandemic, and swine).
Applications
WB, IP, IF, IHC, ELISA
Recommander des dilutions
WB: 1:300-1:1,000, IP: 1:100, IF: 1:200, IHC: 1:200
Reactivité
Influenza A Virus
Réactivité croisée
This antibody does not react with Influenza B viruses.
Immunogène
Human Influenza A Virus (H2N2) Okada strain.
Hôte
Mouse
Clonalité
Monoclonal
Clone
C43
Isotype
IgG2a
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Pureté
> 90% (by SDS-PAGE)
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibodyCliquez pour ouvrir le point de vue élargi
Figure 1: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HACliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses.Cliquez pour ouvrir le point de vue élargi
Figure 3: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.Cliquez pour ouvrir le point de vue élargi
Figure 4: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody

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