This antibody recognises human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.
Applications
WB, ELISA
Recommander des dilutions
ELISA: 0.15 - 0.30 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivité
Human
Immunogène
Recombinant human IL-7.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Biotin
Concentration
100 µg/ml after reconstitution.
Forme du produit
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Stockage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Notes générales
Rabbit anti Human Interleukin-7 antibody recognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.