This antibody recognises mouse interleukin-21 (IL-21), a pleiotropic cytokine and member of the IL-15/IL-21 family, secreted by stimulated CD4+ T cells, which enhances stimulated B-cell proliferation/differentiation, the proliferation of bone marrow progenitor cells, and increases the activity of NK cells and disease-specific CD8+ T-cells.IL-21 signals through binding to the specific type I cytokine receptor IL-21R, coupled with the common cytokine receptor g chain (gc), initiating the activation of the JAK/STAT signalling pathway.
Applications
WB, ELISA
Recommander des dilutions
ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivité
Mouse
Immunogène
Recombinant mouse IL-21.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Biotin
Concentration
100 µg/ml after reconstitution.
Forme du produit
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Stockage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Notes générales
Rabbit anti Mouse Interleukin-21 antibody recognizes mouse interleukin-21 (IL-21), a pleiotropic cytokine and member of the IL-15/IL-21 family, secreted by stimulated CD4+ T cells, which enhances stimulated B-cell proliferation/differentiation, the proliferation of bone marrow progenitor cells, and increases the activity of NK cells and disease-specific CD8+ T-cells.IL-21 signals through binding to the specific type I cytokine receptor IL-21R, coupled with the common cytokine receptor g chain (gc), initiating the activation of the JAK/STAT signalling pathway.
