Anti-IL-18 Anticorps (A83377)

Name: Anti-IL-18 Antibody
Brand: Antibodies.com
SKU: A83377
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab106939). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre IL-18 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-IL-18 Antibody

Description du produit

Goat polyclonal antibody to IL-18.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:32,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human IL-18.

Séquence

C-NPPDNIKDTKSDI

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

23 kDa

MW prédit

22.3kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Iboctadekin, IFN gamma inducing factor, IFN-gamma-inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL-1 gamma, IL1 gamma, IL18, IL18 protein, IL18_HUMAN, IL1F4, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interferon gamma-inducing factor, Interleukin 1 gamma, Interleukin 18, Interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, Interleukin-18, Interleukin18, MGC12320

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-IL-18 Antibody (A83377)

IL-18 expression in HeLa (A), A431 (B), and US0S (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-IL-18 Antibody (A83377) at 0.1µg/ml (A) or 2µg/ml (B-C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-IL-18 Antibody (A83377)

IL-18 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-IL-18 Antibody (A83377)

IL-18 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, Golgi apparatus, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-IL-18 Antibody (A83377)

IL-18 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.