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IKK alpha (phospho Thr23) Cell Based Kit Elisa (A102584)

Nom du produit
IKK alpha (phospho Thr23) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This IKK alpha (phospho Thr23) Cell Based ELISA Kit allows for the detection of IKK alpha (phospho Thr23) and the effects that certain stimulation conditions have on IKK alpha (phospho Thr23) expression in different cell lines. Qualitative determination of IKK alpha (phospho Thr23) concentration is achieved by an indirect ELISA format. In essence, the IKK alpha (phospho Thr23) is captured by Anti-IKK alpha (phospho Thr23) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IKK alpha (phospho Thr23) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-IKK alpha Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
chuk, CHUK1, Conserved Helix Loop Helix Ubiquitous Kinase, Conserved helix loop ubiquitous kinase, Conserved helix-loop-helix ubiquitous kinase, I Kappa B Kinase 1, I Kappa B Kinase Alpha, I-kappa-B kinase 1, I-kappa-B kinase alpha, IkappaB kinase, IkB kinase alpha subunit, IkBKA, IKK 1, IKK A, IKK a kinase, IKK-A, IKK-alpha, IKK1, IKKA, IKKA_HUMAN, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear Factor Kappa B Inhibitor Kinase Alpha, Nuclear factor NF kappa B inhibitor kinase alpha, Nuclear factor NF-kappa-B inhibitor kinase alpha, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor, TCF-16, TCF16, Transcription factor 16
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585)
Western blot analysis of extracts from MDA-MB-435 cells treated with EGF, using Anti-IKK-alpha (phopho Thr23) Antibody.
Protocol Diagram - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585)
A diagram showing the protocol for IKK alpha (phospho Thr23) Cell Based ELISA Kit.
Protocol Summary - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585)
A summary of the protocol for IKK alpha (phospho Thr23) Cell Based ELISA Kit.
Western Blot - Control Antibody - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585)
The mouse monoclonal antibody to GAPDH is used as a positive control inIKK alpha (phospho Thr23) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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